Table 2.

Side-by-side comparison of values for constants obtained with the presented method and an independent optical label-free technique of spectral-correlation interferometry

Method	Target	Interaction	kon, M−1s−1	koff, s−1	KA, M−1	KD, M
Label-free SCI	cTnI	TBB–target	(7.9 ± 0.8) × 104	(2.5 ± 0.4) × 10−4	(3.1 ± 0.6) × 108	(3.2 ± 0.6) × 10−9
Proposed here	cTnI	TBB–target	(7.3 ± 1.0) × 104	(2.9 ± 0.2) × 10−4	(2.5 ± 0.4) × 108	(4.0 ± 0.6) × 10−9
Proposed here	cTnI	MPB–target	(6.8 ± 1.4) × 107	(2.8 ± 1.2) × 10−7	(2.4 ± 1.2) × 1014	(4.1 ± 2.0) × 10−15
Label-free SCI	SEB	TBB–target	(1.5 ± 0.3) × 104	(3.3 ± 1.0) × 10−4	(4.6 ± 1.7) × 107	(2.2 ± 0.8) × 10−8
Proposed here	SEB	TBB–target	(8.4 ± 0.9) × 103	(2.4 ± 0.3) × 10−4	(3.4 ± 0.6) × 107	(2.9 ± 0.5) × 10−8
Proposed here	SEB	MPB–target	(1.1 ± 0.3) × 107	(3.0 ± 1.7) × 10−7	(3.5 ± 2.2) × 1013	(2.8 ± 1.7) × 10−14