Fig. 2. Effect of GH on TG accumulation and adipocyte differentiation in SVCs.

Cells were incubated with GH (5 or 15 ng/mL) in differentiation medium for 2 wk. Untreated cells (0 ng/mL GH, no MDI) were used as negative controls, and MDI-only-treated cells were used as positive controls. The MDI mixture comprised 1 µM dexamethasone (DEX), 0.5 mM isobutylmethylxanthine (IBMX), and 10 µg/mL insulin (INS). (A) Differentiated cells were stained with Oil Red O and visualized under a microscope. (B) Oil Red O staining was performed and quantified to measure TG accumulation. Values are expressed as a percentage of that in the negative controls, and each value represents the mean ± SD (n = 3). Superscripts indicate statistically significant differences (p< 0.05). MDI, methylene diphenyl diisocyanate; GH, growth hormone; TG, triglyceride; SVCs, stromal vascular cells.