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Oligonucleotide sequences utilized for recognition of Stx1, Stx2, and eae virulence genes of E. coli from mastitic milk.
| Target gene | Primer sequences (5ʹ–3ʹ) | Base pair |
|---|---|---|
| stx1 | CAGTTAATGTGGTGGCGAAGG | 348 |
| CACCAGACAATGTAACCGCTG | ||
| Stx2 | ATCCTATTCCCGGGAGTTTACG | 584 |
| GCGTCATCGTATACACAGGAGC | ||
| eae | TCAATGCAGTTCCGTTATCAGTT | 482 |
| GTAAAGTCCGTTACCCCAACCTG |
