Figure 1.

Experimental timeline with dosing regimen and terminal analysis. After habituation and baseline (BL) measures for behavior, all mice were subjected to intraperitoneal injection of paclitaxel (4 mg/kg per mouse) after BL i.e. on day 0, D2, D4, and D6 to induce peripheral neuropathy. Behavioral assays (mechanical hypersensitivity, grimace, grip strength, and thermal hypersensitivity) were measured on BL, D1, D3, D5, D7, and D9. On D10, animals were terminated and spinal cord, popliteal and inguinal lymph nodes, and DRG (L3 to T13) were collected. The lumbar portion of the spinal cord was used for immunohistochemical analyses for glia reactivity. The lymph nodes and DRG were used for flow cytometry analyses of immune cell populations. DRG neurons were plated and used for Seahorse analyses of bioenergetics.