Figure 3.

Paclitaxel dysregulates CD4⁺ and CD8⁺ T-cell subpopulations in an eIF4E-dependent manner. (A) Gating strategy used for flow cytometry of lymphocytes from popliteal and inguinal lymph nodes. T-cells were separated from the whole lymphoid cells population using CD3. CD3⁺ cells were then gated for CD4⁺ T-cell and CD8⁺ T-cells. For each of these subsets, cells were further gated for CCR7 or CD25 and CD44. (B) Quantification of CD4⁺ T-cells from the CD3 population from male mice. (C) CCR7⁺ T-cells from isolated CD4⁺ population in males, **p 0.0063. (D) CD4⁺ cells gated for CD25 and/or CD44 in males. (E) Quantification of CD4⁺ T-cells from the CD3⁺ population from female mice, *p 0.0101. (F) CCR7⁺ T-cells from isolated CD4⁺ population in female mice, *p 0.0250. (G) CD4⁺ cells gated for CD25 and/or CD44 in female mice, **p 0.0011. (H) Quantification of CD8⁺ T-cells from the CD3⁺ population from male mice. (I) CCR7⁺ T-cells from isolated CD8⁺ population in males, *p 0.0433. (J) CD8⁺ cells gated for CD25 and/or CD44 in males, *p 0.0396. (K) Quantification of CD8⁺ T-cells from the CD3 population from female mice, *p 0.041. (L) CCR7⁺ T-cells from isolated CD8⁺ population in female mice, **p 0.0097. (M) CD8⁺ cells gated for CD25 and/or CD44 in female mice. All data are presented as mean ± standard error of the mean, n = 3 mice each for male/female WT vehicle-treated and WT paclitaxel groups, n = 4 mice each for male/female eIF4E^S209A vehicle-treated and paclitaxel. Two-way ANOVA was performed with Tukey’s post-hoc for multiple comparisons.