Figure 4.

Paclitaxel induces activation of antigen-presenting myeloid cells in eIF4E^S209A mice DRG. (A) Gating strategy for isolating myeloid APCs from mouse DRG. Total cells were gated for CD11b and CD45 to separate out immune cells. The CD11b⁺ CD45⁺ subset was then gated for MHC-II⁺ and/or CD40⁺ cells. (B) Quantification of CD40⁺ and/or MHC-II⁺ cells from male mice, *p 0.0275. (C) Quantification of CD40⁺ and/or MHC-II⁺ cells from female mice, *p 0.014, **p 0.0055, ****p<0.0001. All data are presented as mean ± standard error of the mean, n = 3 mice each for male/female WT vehicle-treated and WT paclitaxel groups, n = 4 mice each for male/female eIF4E^S209A vehicle-treated and paclitaxel. Two-way ANOVA was performed with Tukey’s post-hoc for multiple comparisons.