Table 1.
Antibody | Company | Catalog number | Working dilution |
---|---|---|---|
Antibodies used for IHC | |||
Anti-Iba1 | WAKO | 019-19741 | 1:1000 |
Anti-GFAP | DAKO | Z-0331 | 1:1000 |
Anti-NeuN | EMD Millipore | MAB377 | 1:1000 |
Anti-ATF3 | Abcam | ab207434 | 1:1000 |
Goat anti-mouse Alexa Fluor 488 | Invitrogen | A21121 | 1:500 |
Goat anti-rabbit Alexa Fluor 647 | Invitrogen | A21245 | 1:500 |
Antibodies used for flow cytometry | |||
Anti-CD16/32 | eBioscience | 16016185 | 1:2000 |
Anti-CD3 Alexa fluor 700 conjugate | eBioscience | 56003280 | 1:200 |
Anti-CD4 Fluorescein isothiocyanate conjugate | eBioscience | 11004185 | 1:200 |
Anti-CD8 Phycoerythrin conjugate | eBioscience | 12-0081-83 | 1:200 |
Anti-CD25 eFluor 450 conjugate | eBioscience | 48025182 | 1:200 |
Anti-CD44 eFluor780 conjugate | eBioscience | 47044182 | 1:200 |
Anti-CCR7 Allophycocyanin conjugate | eBioscience | 17197942 | 1:200 |
Anti-CD11b Allophycocyanin-Cy7 conjugate | Life Technologies | A15390 | 1:200 |
Anti-CD45 Brilliant violet 421 conjugate | Biolegend | 103133 | 1:200 |
Anti-MHCII Alexa fluor 488 conjugate | eBioscience | 11532282 | 1:2000 |
Anti-CD40 Phycoerythrin conjugate | eBioscience | 120401-82 | 1:200 |