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. 2021 Apr 12;12:642420. doi: 10.3389/fimmu.2021.642420

Table 1.

Antibodies used for IHC and flow cytometry.

Antibody Company Catalog number Working dilution
Antibodies used for IHC
Anti-Iba1 WAKO 019-19741 1:1000
Anti-GFAP DAKO Z-0331 1:1000
Anti-NeuN EMD Millipore MAB377 1:1000
Anti-ATF3 Abcam ab207434 1:1000
Goat anti-mouse Alexa Fluor 488 Invitrogen A21121 1:500
Goat anti-rabbit Alexa Fluor 647 Invitrogen A21245 1:500
Antibodies used for flow cytometry
Anti-CD16/32 eBioscience 16016185 1:2000
Anti-CD3 Alexa fluor 700 conjugate eBioscience 56003280 1:200
Anti-CD4 Fluorescein isothiocyanate conjugate eBioscience 11004185 1:200
Anti-CD8 Phycoerythrin conjugate eBioscience 12-0081-83 1:200
Anti-CD25 eFluor 450 conjugate eBioscience 48025182 1:200
Anti-CD44 eFluor780 conjugate eBioscience 47044182 1:200
Anti-CCR7 Allophycocyanin conjugate eBioscience 17197942 1:200
Anti-CD11b Allophycocyanin-Cy7 conjugate Life Technologies A15390 1:200
Anti-CD45 Brilliant violet 421 conjugate Biolegend 103133 1:200
Anti-MHCII Alexa fluor 488 conjugate eBioscience 11532282 1:2000
Anti-CD40 Phycoerythrin conjugate eBioscience 120401-82 1:200