. 2021 Apr 12;12:642420. doi: 10.3389/fimmu.2021.642420

Table 4.

Statistical values for DRG neuron mitochondrial respiration.

Dataset	Main effect		Interaction		Multiple comparisons
Dataset	F (DFn,DFd)	p-value	F (DFn,DFd)	p-value	Effect	Groups	p-value
Males OCR AUC	Treatment: F (1, 11) = 11.85	0.0055	F (1, 11) = 5.817	0.0345	Treatment	Vehicle	0.5347
	Treatment: F (1, 11) = 11.85	0.0055			Treatment	Paclitaxel	0.2303
	Genotype: F (1, 11) = 0.1714	0.6868			Genotype	WT	0.0136
	Genotype: F (1, 11) = 0.1714	0.6868			Genotype	eIF4E^S209A	0.8475
Females OCR AUC	Treatment: F (1, 17) = 6.074	0.0247	F (1, 17) = 5.158	0.0364	Treatment	Vehicle	0.7519
	Treatment: F (1, 17) = 6.074	0.0247			Treatment	Paclitaxel	0.1426
	Genotype: F (1, 17) = 0.6271	0.4393			Genotype	WT	0.0455
	Genotype: F (1, 17) = 0.6271	0.4393			Genotype	eIF4E^S209A	0.9982
Males ECAR AUC	Treatment: F (1, 11) = 13.04	0.0041	F (1, 11) = 16.26	0.0020	Treatment	Vehicle	0.1313
	Treatment: F (1, 11) = 13.04	0.0041			Treatment	Paclitaxel	0.0303
	Genotype: F (1, 11) = 0.2927	0.5993			Genotype	WT	0.0021
	Genotype: F (1, 11) = 0.2927	0.5993			Genotype	eIF4E^S209A	0.9866
Females ECAR AUC	Treatment: F (1, 17) = 7.989	0.0116	F (1, 17) = 2.068	0.1686	Treatment	Vehicle	0.6269
	Treatment: F (1, 17) = 7.989	0.0116			Treatment	Paclitaxel	0.8523
	Genotype: F (1, 17) = 0.1287	0.7242			Genotype	WT	0.0792
	Genotype: F (1, 17) = 0.1287	0.7242			Genotype	eIF4E^S209A	0.6287
Breakdown datasets for OCR	Interaction	p-value	Multiple comparisons - groups compared				p-value
Males basal respiration	F (3, 8) = 233.2	<0.0001	WT vehicle		WT paclitaxel		<0.0001
			eIF4E^S209A vehicle		eIF4E^S209A paclitaxel		0.0134
			WT vehicle		eIF4E^S209A vehicle		0.0018
			WT paclitaxel		eIF4E^S209A paclitaxel		<0.0001
Females basal respiration	F (3, 8) = 838.7	<0.0001	WT vehicle		WT paclitaxel		<0.0001
			eIF4E^S209A vehicle		eIF4E^S209A paclitaxel		<0.0001
			WT vehicle		eIF4E^S209A vehicle		0.8413
			WT paclitaxel		eIF4E^S209A paclitaxel		<0.0001
Males ATP turnover	F (3, 12) = 2.384	0.1203	WT vehicle		WT paclitaxel		0.0905
			eIF4E^S209A vehicle		eIF4E^S209A paclitaxel		0.9832
			WT vehicle		eIF4E^S209A vehicle		0.9188
			WT paclitaxel		eIF4E^S209A paclitaxel		0.5864
Females ATP turnover	F (3, 12) = 8.828	0.0023	WT vehicle		WT paclitaxel		0.0021
			eIF4E^S209A vehicle		eIF4E^S209A paclitaxel		0.6736
			WT vehicle		eIF4E^S209A vehicle		0.9766
			WT paclitaxel		eIF4E^S209A paclitaxel		0.0481
Males maximal respiration	F (3, 28) = 2.233	0.1064	WT vehicle		WT paclitaxel		0.0661
			eIF4E^S209A vehicle		eIF4E^S209A paclitaxel		0.9857
			WT vehicle		eIF4E^S209A vehicle		0.8539
			WT paclitaxel		eIF4E^S209A paclitaxel		0.6683
Females maximal respiration	F (3, 28) = 5.986	0.0028	WT vehicle		WT paclitaxel		0.0011
			eIF4E^S209A vehicle		eIF4E^S209A paclitaxel		>0.9999
			WT vehicle		eIF4E^S209A vehicle		0.4836
			WT paclitaxel		eIF4E^S209A paclitaxel		0.0417
Males non-mitochondrial respiration (reserve capacity)	F (3, 8) = 42.18	<0.0001	WT vehicle		WT paclitaxel		<0.0001
			eIF4E^S209A vehicle		eIF4E^S209A paclitaxel		0.9978
			WT vehicle		eIF4E^S209A vehicle		0.0063
			WT paclitaxel		eIF4E^S209A paclitaxel		0.0010
Females non-mitochondrial respiration (reserve capacity)	F (3, 8) = 46.31	<0.0001	WT vehicle		WT paclitaxel		<0.0001
			eIF4E^S209A vehicle		eIF4E^S209A paclitaxel		0.0074
			WT vehicle		eIF4E^S209A vehicle		0.0037
			WT paclitaxel		eIF4E^S209A paclitaxel		0.9212

Two-way ANOVA was performed on AUC values for OCR and ECAR followed by Tukey’s post-hoc for multiple comparisons between genotypes and treatments for each sex. For breakdown data of mitochondrial respiration, ordinary one-way ANOVAs were used to analyze mean values of four groups (WT vehicle, WT paclitaxel, eIF4E^S209A vehicle, and eIF4ES^209A paclitaxel), followed by Sidak’s post-hoc for multiple comparisons between genotypes and treatments for each sex. p ≤ 0.05 was considered significant (bolded). OCR, oxygen consumption rate; ECAR, extracellular acidification rate.