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. 2021 Apr 12;12:659996. doi: 10.3389/fimmu.2021.659996

Figure 2.

Figure 2

TCM derived from serum deprived TNBC cell lines retain neutrophil recruiting ability. (A) Flowchart describing how TCM was generated and harvested from cell lines grown under serum free conditions. (B) Graph depicting spontaneous LDH activity in the supernatant of cancer cell lines grown under serum free conditions measured and normalized with respect to maximum LDH activity. Bars represent mean percentage of max LDH activity ± SEM from 3 independent experiments. (C) Graph depicting the percentage of neutrophils that migrated into the bottom chamber of the transwells containing equal volume of TCM from different breast cancer cell lines cultured in serum free condition. Red and blue bars represent values of neutrophil migration obtained with TCM harvested from TNBC and ER+ cancer cell lines, respectively. (D) Graph depicting the percent of neutrophils migrating into the bottom chamber of the transwells containing serially diluted equal volume of TCM from two different TNBC cell lines. Results are presented as mean ± SEM from N=3-7 (C) or N=3 (D) independent donors represented by individual dots. Each donor (N) represents an independent experiment. **P ≤ 0.005, *P ≤ 0.05 when compared with MCF-7 (one-way ANOVA with Dunnett’s multiple comparisons test).