FIGURE 2.

Triptolide treatment modulates the expression of lncRNA-associated pro-inflammatory genes Ptgs-2 and IL-6 in resting monocyte-derived macrophages (MDMs). Concentration dependence (A, C): MDMs were treated with increasing concentrations of triptolide for 6 and 24 h. DMSO was used as a vector control. Total RNA was extracted, and then Ptgs-2 (A) and IL-6 (C) expression levels were assessed by RT-qPCR. Time dependence (B, D): MDMs were treated with 100 nM triptolide for 6, 12, and 24 h. DMSO was used as a vector control. Total RNA was extracted, and then Ptgs-2 (B) and IL-6 (D) expression levels were assessed by RT-qPCR. Relative expression was calculated using the $-\Delta \Delta Ct$ method. *p < 0.05; **p < 0.01; ***p < 0.001.