FIGURE 5.

Triptolide treatment decreases Mtb HN878 growth in MDMs but not in the culture medium. (A): Graphical summary for the experiment on the effect of triptolide on Mtb HN878 growth within MDMs. Triptolide reduces Mtb HN878 CFU in MDMs (B): 10^5 MDMs were infected with Mtb HN878 MOI:2 for 4 h then treated with 100 nM triptolide for 24 and 48 h. DMSO was used as vector control. At each time point, BMDMs were lysed and the lysate plated for determination of CFU 15 days later. Triptolide does not affect Mtb HN878 growth in culture medium (C): 2 × 10^5 Mtb HN878 CFU was seeded on 96-well plates and then treated with triptolide, rifampicin, or mixtures of both at different concentrations. DMSO was used as a vector control. alamarBlue stain was added to the plate on day 14 and OD was read at 570 nm *p < 0.05; **p < 0.01; ***p < 0.001.