Figure 1.

Generation of a Heterogeneous Population of Human Induced Neurons

(A) Overview of the single-cell TF multiplex pipeline.

(B) Immunostaining for canonical neuronal marker genes of fibroblasts at day 0 and CHi and TFi at 9 and 21 dpi. Scale bars, 100 μm. YFP (green) marks infected cells and cell nuclei were visualized using DAPI nuclear stain (gray).

(C) Quantification of immunostainings in (B). n = 4 independent experiments, unpaired Student's t test. Error bars represent mean + SD.

(D) qPCR for pan-neuronal marker genes (MAP2, NRCAM, NEUN, SYN1; top), canonical neuronal subtype markers (SLC17A7, GABRA1, TH, CHAT; middle), and canonical fibroblast markers (VIM, SNAI1; bottom). n = 3 independent experiments, unpaired Student's t test. Error bars represent mean + SD.

(E) Immunostainings for canonical neuronal subtype markers (red) of TFi at 21 dpi. Scale bars, 100 μm. YFP (green) marks infected cells and cell nuclei were visualized using DAPI nuclear stain (gray).

(F) Quantification of immunostainings in (E). n = 4 independent experiments, unpaired Student's t test. Error bars represent mean + SD.