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. 2021 Apr 12;12:624404. doi: 10.3389/fphar.2021.624404

FIGURE 4.

FIGURE 4

Yiqi Jiedu Huayu decoction (YJHD) improves renal fibrosis. (A) Masson staining was used to analyze collagen fibers in the kidney, and immunohistochemistry was used to analyze the expression of fibronectin and collagen IV. In the diabetic group, collagen fibers deposition (arrows) was observed in Masson staining. (B) ImageJ software was used to calculate the degree of renal fibrosis. (C, D) Image-Pro Plus software was used to statistically analyze immunohistochemical staining results of fibronectin and collagen IV, respectively. (E) Protein levels of fibronectin and collagen IV in the kidney were detected by western blotting. (F, G) Protein concentration analysis. (H, I) mRNA expression levels of fibronectin and collagen IV in kidneys were detected by PCR. Data are expressed as *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001: compared with the normal control group; #p < 0.05, ##p < 0.01, ###p < 0.001, and ####p < 0.0001: compared with the diabetic group, respectively.