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. 2021 Apr 12;11:656078. doi: 10.3389/fonc.2021.656078

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Copyright © 2021 Mo, He, Zheng, Chen, Tang and Yan

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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tRF-17-79MP9PP is a type of tRNA-derived fragment. (A) tRF-17-79MP9PP is a 5’- tRF fragment, with 18 genomic locations. (B) tRF-17-79MP9PP was derived from 5’- ends of mature tRNA-Val-AAC and tRNA-Val-CAC with the length of 17nt. The tRF-17-79MP9PP sequences are in the textbox and the arrow points to the cutting position. (C) The dissolution curve and amplification curve of tRF-17-79MP9PP. (D) The product of RT-PCR was run on 2% agarose gel. (E) The product of qRT-PCR was confirmed by Sanger sequencing. (F) Nucleocytoplasmic separation assay revealed that tRF-17-79MP9PP is mainly expressed in the cytoplasm. SONRD44 and actin were used as nuclear and cytoplasm localization markers, respectively.