Figure 1.

Determination of SARS-CoV and SARS-CoV-2 infected hMDM by immunolabeling for dsRNA and N protein. Vere E6 cells were infected with SARS-CoV and SARS-CoV-2 at MOI 1 TCID₅₀/cell, and after 24 hpi dsRNA and N protein were labeled with specific antibodies. The nuclei were stained with DAPI. Then positive cells for dsRNA and N were quantified either by flow cytometry or immunofluorescence microscopy (A). In (B) example of representative images acquired by fluorescence microscopy is shown. The scale bar represent 40 µm. The data are from three independent experiments. Statistically significant differences between the conditions are indicated by asterisks (ns indicates non-statistical differences, *p < 0.05, **p ≤ 0.002 and ****p ≤ 0.0001).