Table 2.
Primer sequences used for real-time quantitative PCR1.
| Gene2 | Primer sequence3 (5’-3’) | Annealing temperature | GeneBank accession number |
|---|---|---|---|
| IFN-α | F: ATCCTGCTGCTCACGCTCCTTCT R: GGTGTTGCTGGTGTCCAGGATG |
64 | AB021154 |
| IFN-β | F: GCCTCCAGCTCCTTCAGAATACG R: CTGGATCTGGTTGAGGAGGCTGT |
64 | AY974089 |
| IFN-γ | F: ACACTGACAAGTCAAAGCCGCACA R: AGTCGTTCATCGGGAGCTTGGC |
60 | X99774 |
| IL-1β | F: GTGAGGCTCAACATTGCGCTGTA R: TGTCCAGGCGGTAGAAGATGAAG |
64 | Y15006 |
| IL-2 | F: TGCAGTGTTACCTGGGAGAAGTGGT R: ACTTCCGGTGTGATTTAGACCCGT |
60 | NM_204153.2 |
| IL-6 | F: CGTGTGCGAGAACAGCATGGAGA R: TCAGGCATTTCTCCTCGTCGAAGC |
60 | NM_204628.1 |
| IL-8 | F: CCAAGCACACCTCTCTTCCA R: GCAAGGTAGGACGCTGGTAA |
64 | AJ009800 |
| IL-12p40 | F: CCAAGACCTGGAGCACACCGAAG R: CGATCCCTGGCCTGCACAGAGA |
60 | AY262752.1 |
| IL-13 | F: ACTTGTCCAAGCTGAAGCTGTC R: TCTTGCAGTCGGTCATGTTGTC |
60 | AJ621250.1 |
| IL-18 | F: GAAACGTCAATAGCCAGTTGC R: TCCCATGCTCTTTCTCACAACA |
64 | AY628648.2 |
| TGF-β4 | F: CGGCCGACGATGAGTGGCTC R: CGGGGCCCATCTCACAGGGA |
60 | M31160.1 |
| β-Actin | F: CAACACAGTGCTGTCTGGTGGTA R: ATCGTACTCCTGCTTGCTGATCC |
58 | X00182 |
1The listed oligonucleotides were used to analyze gene expression via real-time quantitative PCR.
2IFN, Interferon; IL, Interleukin.
3F, forward; R, reverse.
4TGF-β, transforming growth factor beta.