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. 2021 Apr 12;12:653755. doi: 10.3389/fimmu.2021.653755

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Copyright © 2021 Wang, Chen, He, Zheng, Tian, Zhao, Elsheikha and Zhu

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Validation of the expression of representative differentially expressed mRNAs (HDAC1, MCM2, CD47) (A), miRNAs [16a-3p (chi-miR-16a-3p), 211 (chi-miR-211), 155-3p (chi-miR-155-3p)] (B), circRNAs [7223 (novel_circ_0017223), 7701 (novel_circ_0007701), 3459 (novel_circ_0003459)] (C), and lncRNAs [5943 (ENSCHIT00000005943), 2500 (LNC_012500), 2081 (ENSCHIT00000002081)] (D) using qRT-PCR. The x-axis shows the names of the selected RNAs and the y-axis shows the relative expression levels. The GADPH gene was used for mRNA, circRNAs, and lncRNA normalization, while the U6 gene was used for miRNA’s normalization.