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. 2021 Apr 12;12:653755. doi: 10.3389/fimmu.2021.653755

Table 1.

qRT-PCR primers used in this study.

Target Gene Forward primer (5′-3′) Reverse primers (5′-3′)
HDAC1 CAGGATCCTTATCGTTGACTGG CGGTGCCAGGAGAAGTAAAG
MCM2 TGGCCAAGAAGGACAACAA AGCGATGCTGGCAAAGAT
CD47 CCTCCTACACCATCAGCATAC GACCATGCACTGGGATACAT
GAPDH* CACCACACCTTCTACAAC TCTGGGTCATCTTCTCAC
chi-miR-16a-3p CGCGCGCGAAATTATCTCCAGTATTA
chi-miR-211 CCTTCCCTTTGTCATCCTTTGCCC
chi-miR-155-3p CTGGGTCTGGCTCCTACATGTT
U6* CAAGGATGACACGCAAATTCG
novel_circ_0017223 CACCTGTGCCGAGAGCTGTA CCTGGGCAGCTGGTTTCTGA
novel_circ_0007701 GAAGAAGCTGCCATGAGAAGAG TGCTGAAGCTGCCGAATATC
novel_circ_0003459 AGATGCTCGCTGCTGCTCTC ATGAGACGTACGTGTCCCGG
ENSCHIT00000005943 TCAGTGTGCGGTTGACTTAG CAACCCTCTGCCTTCTCAAA
LNC_012500 CGTAACGCGAATCGCAAAC TGAGGATCGGGTTAAGGGT
ENSCHIT00000002081 AGACTGTGTCCAGAGGTAACTA GCTGTGAAAGATGGTGAGTAAATG

*The GADPH gene was used for mRNA, circRNA and lncRNA normalization, while the U6 gene was used for miRNA’s normalization.