Table 1.
Target Gene | Forward primer (5′-3′) | Reverse primers (5′-3′) |
---|---|---|
HDAC1 | CAGGATCCTTATCGTTGACTGG | CGGTGCCAGGAGAAGTAAAG |
MCM2 | TGGCCAAGAAGGACAACAA | AGCGATGCTGGCAAAGAT |
CD47 | CCTCCTACACCATCAGCATAC | GACCATGCACTGGGATACAT |
GAPDH* | CACCACACCTTCTACAAC | TCTGGGTCATCTTCTCAC |
chi-miR-16a-3p | CGCGCGCGAAATTATCTCCAGTATTA | |
chi-miR-211 | CCTTCCCTTTGTCATCCTTTGCCC | |
chi-miR-155-3p | CTGGGTCTGGCTCCTACATGTT | |
U6* | CAAGGATGACACGCAAATTCG | |
novel_circ_0017223 | CACCTGTGCCGAGAGCTGTA | CCTGGGCAGCTGGTTTCTGA |
novel_circ_0007701 | GAAGAAGCTGCCATGAGAAGAG | TGCTGAAGCTGCCGAATATC |
novel_circ_0003459 | AGATGCTCGCTGCTGCTCTC | ATGAGACGTACGTGTCCCGG |
ENSCHIT00000005943 | TCAGTGTGCGGTTGACTTAG | CAACCCTCTGCCTTCTCAAA |
LNC_012500 | CGTAACGCGAATCGCAAAC | TGAGGATCGGGTTAAGGGT |
ENSCHIT00000002081 | AGACTGTGTCCAGAGGTAACTA | GCTGTGAAAGATGGTGAGTAAATG |
*The GADPH gene was used for mRNA, circRNA and lncRNA normalization, while the U6 gene was used for miRNA’s normalization.