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. 2021 Apr 12;8:656047. doi: 10.3389/fmed.2021.656047

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Copyright © 2021 Delbue, Lebenheim, Cardoso-Silva, Dony, Krug, Richter, Manna, Muñoz, Wolk, Heldt, Heimesaat, Sabat, Siegmund and Schumann.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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IL-22 induces development of dyspolar 3dimensional cysts. CaCo-2 cells were seeded in Matrigel and 3D-cysts were allowed to develop within 5–7 days. Then cysts were fixed and immunostained according to the Methods section. (A–C) control 3D-cysts. (D–F) cysts treated with IL-22 (10 ng/ml) starting at the day after seeding. Proteins detected by immunostaining are depicted in each image. The composite image (right column) additionally includes staining for nuclei using DAPI. Structures identified by arrows are explained in the text of the Results chapter; n = 4 independent experiments.