Figure 5.

IL-22 affects tight junction proteins. (A) Expression of TJ proteins: IECs were treated with IL-22 (10 ng/ml) for the indicated times. Subsequently, cells were lysed. TJ protein levels were determined by Western blotting as explained in the Methods section. Representative blots of three independent experiments. (B–D) Confocal LSM after immunostaining of CaCo-2 cells. (B) Reduction of JAM-A junctional levels by IL-22. Red, JAM-A; green, ZO-1; blue, nuclei n = 3. IECs cartoon represents the confocal microscopy analysis (C,D) Dislocation of occludin by IL-22. Green, occludin; red, actin; blue, nuclei; n = 3.