Figure 7.

MAPK/ERK signaling pathway is pivotal for IL-22 barrier signaling in IECs. HT-29/B6 cells were exposed to U0196 (2 h) in the presence or absence of IL-22 (1 h). (A) Protein levels of ERK and phospho-ERK were assessed by Western blotting. Representative blots of three independent experiments are shown. (B) TER of HT-29/B6 cells were measured after treatment with U0126 and IL-22 (10 ng/ml), as depicted; n = 9, ***p < 0.001. (C) Confocal LSM after immunostaining of occludin was performed. Green, occludin; blue, nuclei. Scale bar: 20 μm. Representative images of three independent experiments. (D) Western blotting was performed to quantify protein levels of E-cadherin, claudin-1,−2,−4, and MMP-7. Representative blots of two independent experiments are shown; ***p < 0.001.