Fig. 8.

TLR4 deficiency reduces CCRL2 expression in TAM and anti-tumor T-cell responses in murine melanoma. (A) Tumor growth curve. (B–E) Surface CCRL2 levels in macrophages (B), CD4⁺ and CD8⁺ T cells were gated and examined for expression of Ki67 (C) and IFN-γ (D), as well as expression of Granzyme B in CD8⁺ T cells (E) from tumors of WT and Tlr4^−/− mice on day 14. (F) Schematic representation of the functional role of CCRL2 in macrophages to potentiate anti-tumor immunity. Data represent mean ± SEM (n = 5 to 6). **P < 0.01; ***P < 0.001.