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. 2021 Apr 14;118(16):e2022841118. doi: 10.1073/pnas.2022841118

Fig. 3.

Fig. 3.

IL-6 is an upstream regulator of FA-mediated hepatic FGF23 production. (A) Violin plot on the association between tertiles of IL-6 and FGF23 in patients at high risk for AKI. (B) Plasma IL-6 level measured by ELISA in mice treated with FA in time-dependent manner (0, 6, and 12 h n = 4 and 24 h n = 5). (C) Plasma IL-6 level measured in C57BL/6J mice injected with control antibody or IL-6 neutralizing (anti–IL-6) antibody in presence or absence of FA for 24 h (n = 5). (D) qPCR analysis of total RNAs isolated from livers of mice injected with control or anti–IL-6 antibody in the presence or absence of FA. (E) Representative images of immunohistochemistry analysis in liver sections of mice injected with control or anti–IL-6 antibody in presence or absence of FA. (F) Plasma intact FGF23 (Left) and C-terminal FGF23 (Right) levels were measured by ELISA in mice injected with control or anti–IL-6 antibody in presence or absence of FA. Data represent mean ± SEM. Data in B was analyzed by two-tailed Student’s t test. Data in C, D, and F were analyzed by one-way ANOVA with Tukey’s multiple comparisons test.