FIGURE 1.

Vascularization of organoids in various methods. (A) The procedure of endothelialized myocardium fabrication using the 3D bio-printing strategy. (B) Schematic illustration of the tissue manufacturing process. (C) Concept schematic of printing a full, thick vascularized tissue in one step. (D) Overview of microfluidic device use for angiogenic sprout inducing in a human lung fibroblast (hLFs) spheroid. Reprinted with permission from Nashimoto et al. (2017). (E) Upon transplantation, host-derived vascular networks invaded the hPSC-derived kidney organoids and connected to the organoid-derived plexus. (F) Brain organoids grown in vitro and transplanted into the mouse cortex.