Table 2.
The chemical analysis used in the experiment.
| Items | Methods | Assay Kits/Testing equipment |
|---|---|---|
| Composition of diets/ingredients | ||
| Moisture | Oven method | Electric blast drying oven (Shanghai Yiheng Scientific Instrument Co., Ltd., Shanghai, China) |
| Protein | Kjeldahl | Auto kieldahl apparatus: Hanon K1100 (Jinan Hanon Instruments Co., Ltd., Jinan, China) |
| Lipid | Soxhlet | Auto fat analy: Hanon SOX606 (Jinan Hanon Instruments Co., Ltd., Jinan, China) |
| Gross energy | Combustion | Oxygen bomb calorimeter: IKA C6000 (IKA WORKS GUANGZHOU, Guangzhou, China) |
| Plasma parameters related antioxidant capacity | ||
| T-SOD1 | WST-1 method | Assay kits purchased from Jian Cheng Bioengineering Institute (Nanjing, China); Spectrophotometer (Thermo Fisher Multiskan GO, Shanghai, China). |
| T-AOC2 | ABTS method | |
| GSH3 | Microplate method | |
| GPx4 | Colorimetric method | |
| MDA5 | TBA method | |
| CAT6 | Ammonium molybdenum acid method | |
| Plasma parameters related ELISA | ||
| IL-107 | Double antibody sandwich method (Test wavelength 450nm) | Assay kits purchased from Jiangsu Enzyme-Free Industrial Co. LTD (Yancheng, China); Thermo Scientific Microplate Reader-1510 (Thermo Fisher Scientific, Shanghai, China). |
| TGF-β8 | ||
| IFN-γ9 | ||
| HSP7010 | ||
| IgM11 | ||
| IgG12 | ||
| C313 | ||
| TNF-α14 | ||
| IL-1β15 | ||
| IL-816 | ||
1T-SOD, total superoxide dismutase; 2T-AOC, total antioxidant capacity; 3GSH, glutathione; 4GPx, glutathione peroxidase; 5MDA, malondialdehyde; 6CAT, catalase; 7IL-10, interleukin 10; 8TGF-β, transforming growth factor-β; 9IFN-γ, interferon-γ; 10HSP70, heat stress protein 70; 11IgM, immunoglobulin M; 12IgG, immunoglobulin G; 13C3, complement component 3; 14TNF-α, tumor necrosis factor-α; 15IL-1β, interleukin 1β; 16IL-8, interleukin 8.