Table 3.
Primer sequence for qRT-PCR.
| Target genes | Forward (5’-3’) | Reverse (5’-3’) | References |
|---|---|---|---|
| β-actin | TCGTCCACCGCAAATGCTTCTA | CCGTCACCTTCACCGTTCCAGT | Ji et al. (25) |
| PI3K1 | AAGAAAGTTTGCCACACCGC | TTGTCCATGGTTCAGTGGCA | Ji et al. (25) |
| Akt2 | GCTGGGTAAAGGCACGTTTG | CTCTCGGTGACCGTATGAGC | Ji et al. (25) |
| NF-κB3 | AGTCCGATCCATCCGCACTA | ACTGGAGCCGGTCATTTCAG | Ji et al. (25) |
| Nrf24 | GGGGAAGTCCTTGAACGGAG | AACCAGCGGGAATATCTCGG | Ji et al. (25) |
| Keap15 | AATATCCGCCGGCTGTGTAG | TGAGTCCGAGGTGTTTCGTG | Ji et al. (25) |
| CAT6 | CAGTGCTCCTGATACCCAGC | TTCTGACACAGACGCTCTCG | Ji et al. (25) |
| Cu/Zn-SOD7 | AGTTGCCATGTGCACTTTTCT | AGGTGCTAGTCGAGTGTTAGG | Ji et al. (25) |
| HO-18 | TCACACCGGGAAACGAGAAG | TGGAGCATTTCTACGGCCAG | Ji et al. (25) |
| HSP709 | CGACGCCAACGGAATCCTAAAT | CTTTGCTCAGTCTGCCCTTGT | Ji et al. (25) |
| Mn-SOD10 | AGCTGCACCACAGCAAGCAC | TCCTCCACCATTCGGTGACA | Ji et al. (25) |
| GPx11 | GAACGCCCACCCTCTGTTTG | CGATGTCATTCCGGTTCACG | Ji et al. (25) |
| TNF-α12 | TGGAGAGTGAACCAGGACCA | AGAGACCTGGCTGTAGACGA | Ji et al. (25) |
| IL-813 | CAGAGAGTCGACGCATTGGT | ATTCACGGTGCTTTGTTGGC | Ji et al. (25) |
| IL-1β14 | TTCTTCCCCTCACCTGGTCT | CCAGCGCGAAGTTTGTCAAT | Ji et al. (25) |
1PI3K, phosphoinositide 3-kinase; 2Akt, protein kinase B; 3NF-κB, nuclear factor-kappa B; 4Nrf2, nuclear factor erythroid 2-related factor 2; 5Keap1, Kelch-like ECH-associated protein 1; 6CAT, catalase; 7Cu/Zn-SOD, copper and zinc superoxide dismutase; 8HO-1, heme oxygenase 1; 9HSP70, heat stress protein 70; 10Mn-SOD, manganese superoxide dismutase; 11GPx, glutathione peroxidase; 12TNF-α, tumor necrosis factor-α; 13IL-8, interleukin 8; 14IL-1β, interleukin 1β.