Figure 5.

Inhibitory effect of FOXP3 knockdown on the invasion and EMT of CaSki and HeLa cells is reversed by LINC00885 overexpression. (A) Invasion assay of CaSki and HeLa cells treated as indicated. After 48 h, the number of cells that had invaded through the membrane were counted under a microscope in five random fields. Magnification, ×200. (B) Expression of EMT-related proteins E-cadherin and Vimentin in CaSki and HeLa cells treated as indicated were detected using western blotting. ***P<0.001 vs. sh-NC; ^#P<0.05, ^##P<0.01, ^###P<0.001 vs. sh-FOXP3 and sh-FOXP3 + pcDNA3.1. EMT, epithelial-mesenchymal transition; sh, short hairpin RNA; FOXP3, forkhead box protein 3; NC, negative control.