Fig. 6.

TRPV4-mediated lowering of IOP is eNOS dependent. (A) Effect of TRPV4 activation on IOP in WT and NOS3^−/− mice. WT and NOS3^−/− animals were administered 5 μL eyedrops of 20 μM GSK101 in one eye and 0.01% DMSO in the contralateral eye 30 min prior to the measurement of dark-adapted IOP. Data are represented as mean ± SEM; ***P < 0.001 versus vehicle group, n = 5 eyes/group; one-way ANOVA followed by Bonferroni’s post hoc test. (B) Dark-adapted baseline IOP in WT C57BL/6J mice and NOS3^−/− mice. Data are represented as mean ± SEM; ***P < 0.001 versus WT, n = 12 eyes/group; unpaired two-tailed t test. n.s., not significant.