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. 2021 Apr 12;11:645682. doi: 10.3389/fonc.2021.645682

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Copyright © 2021 Tang, Wang, Ji, Zheng, Yu, Deng, Zhou and Fang

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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MiR-640 was down-regulated in BC cell lines and tissues. (A, B) MiR-640 had low expression in BC tissues compared with adjacent normal tissues. (C) MiR-640 had low expression in BC cell lines. (D) Detection of colocalization of miR-640 in cytoplasm by FISH assay (magnification, × 400). Red, miR-640; Blue, DAPI. (E, F) Expression levels of cytoplasmic control transcripts (GAPDH), the nuclear control transcript (U6), and miR-640 were determined by RT-qPCR in the cytoplasmic and nuclear fractions of BC cells. ***p < 0.001; ****p < 0.0001.