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. 2021 Apr 12;11:645682. doi: 10.3389/fonc.2021.645682

Figure 4.

Figure 4

Wnt7b is a direct target of miR-640. (A) Putative complementary site within miR-640 and Wnt7b predicted by bioinformatics analysis (TargetScan). (B) Luciferase reporter assay demonstrated that Wnt7b is a direct target of miR-640. (C) Wnt7b mRNA level was determined by RT-PCR in MDA-MB-231 and MCF-7 cells with different treatment. (D–F) Representative Western blotting and quantification of Wnt7b, β-catenin, Gsk-3β, Cyclin D1 and C-myc in MDA-MB-231 and MCF-7 cells with different treatment, β-actin was used as a control. (G, H) Representative Western blotting and quantification of β-catenin in nuclear fraction and cytoplasm fraction of BC cells with different treatment, LaminA was used as a control. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. "ns" means not statistically significant and represents P < 0.05.