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. 2021 Apr 12;11:645682. doi: 10.3389/fonc.2021.645682

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Copyright © 2021 Tang, Wang, Ji, Zheng, Yu, Deng, Zhou and Fang

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Overexpression of miR-640 inhibited BC growth in vivo. (A) Overexpression of miR-640 in MDA-MB-231 cells was verified by RT-qPCR. (B) Representative images of xenograft tumors in nude mice. (C) The growth curves of xenografts. (D) Extract protein from tumors and measuring the expression of Wnt7b by Western blotting. (E) Immunohistochemistry (IHC) staining of Wnt7b in xenografts. (F) The mechanism diagram was generated to illustrate the mechanism of miR-640/Wnt7b/Wnt-β-catenin axis in BC. ****p < 0.0001.