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. 2021 Apr 12;12:649820. doi: 10.3389/fphar.2021.649820

FIGURE 5.

FIGURE 5

The modulation of the Nrf2-mediated antioxidant response by HRF and HSP in mouse dorsal skin irradiated with UVA. Dorsal skin was treated with compounds as previously describes and harvested at 1 h following the final irradiation. Images of immunofluorescence staining (FITC-conjugated secondary antibody staining indicated the location of Nrf2 (green by the anti-Nrf2 antibody. DAPI staining indicated the location of the nucleus (blue) and the merged image indicated the nuclear localization of Nrf2 (A). The summary graph with the statistical analysis of the nuclear-to-cytosolic Nrf2 ratio (E) was quantified by ImageJ and GraphPad prism software and was expressed as mean ± SD, n = 4. The immunofluorescence of antioxidant proteins including GST (B), NQO-1 (C), and the oxidative DNA damage 8-OHdG (D), were collected at 6 h following the final UVA exposure. The summary graph with the statistical analysis of the protein levels of GST (F), NQO-1 (G), and 8-OHdG (H) were quantified by ImageJ and GraphPad prism software and were expressed as mean ± SD, n = 4. ***p < 0.001 vs. non-irradiated sham group by Student’s t-test. #p < 0.05; ##p < 0.01; ###p < 0.001 vs. the sham-irradiated group by one-way ANOVA Dunnett's test.