TABLE 1.

Posttranslational modifications of TFEB *.

Aminoacid Residue	Enzyme	Effect
Ser 3	Phosphorylation by MAP3K3	Inhibit Ser 211 phosphorylation mediated by mTORC1
Ser 122	Phosphorylation by mTOR	Cytosolic retention
Ser 134	Phosphorylation by GSK3	Cytosolic retention
Ser 138	Phosphorylation by GSK3	Cytosolic retention and export from nucleus
Ser 142	Phosphorylation by mTOR, Erk 1/2	Cytosolic retention
Ser 142	Nuclear phosphorylation by CDK4 or CDK6	Nuclear export
Ser 211	Phosphorylation by mTOR	Cytosolic retention and 14-3-3 binding site
Ser 462	Phosphorylation by PKC	Stabilization of TFEB
Ser 463	Phosphorylation by PKC	Stabilization of TFEB
Ser 467	Phosphorylation by PKC	Stabilization of TFEB
Ser 467	Phosphorylation by AKT	Cytosolic retention
Ser 469	Phosphorylation by PKC	Stabilization of TFEB
Lys 116	Deacetylation by Sirtuin-1	Increase of transcriptional activity
Lys 116	Acetylation by GCN5	Suppression of transcriptional activity
Lys 274	Acetylation by GCN5	Suppression of transcriptional activity
Lys 279	Acetylation by GCN5	Suppression of transcriptional activity

*CDK4 and CDK6 also phosphorylate Ser 114 and Thr 331 but without any specific effect (Yin et al., 2020).