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. 2021 Apr 14;118(16):e2016469118. doi: 10.1073/pnas.2016469118

Fig. 2.

Fig. 2.

Growth curves and morphology of H. pylori cultured with various sterols. (A) Growth curves of a standard H. pylori strain (ATCC 43504) cultured in medium supplemented with 150 μM indicated sterols. Each value represents the average of triplicate measurements, and error bars indicate SE. Statistical analysis was performed using cholesterol as a reference (*P < 0.05, **P < 0.01). (B) Morphology of H. pylori (ATCC 43504) cultured with indicated sterols over a 4 d culture period. Immunocytochemistry was performed using an anti-H. pylori antibody (Scale bar, 2 μm). (C) Growth curves of the clarithromycin-resistant strain H. pylori (“2460”) cultured in medium supplemented with 150 μM indicated sterols. Each value represents the average of triplicate measurements, and error bars indicate SE. Statistical analysis was performed using cholesterol as a reference (*P < 0.05). (D) Morphology of H. pylori (“2460”) cultured with indicated sterols over a 4 d culture period. Immunocytochemistry was performed using an anti-H. pylori antibody (Scale bar, 2 μm).