Fig. 4.

Cholestenone treatment reduces CGL expression in H. pylori. (A) Thin layer chromatogram of αCGs isolated from H. pylori (ATCC 43054) cultured with supplements including inactivated horse serum (as a control) or cholesterol or cholestenone, as indicated. (B) Morphology of H. pylori cultured with various supplements. H. pylori (ATCC 43054) was immunostained with anti-CGL antibody (Upper, first three panels) or anti-H. pylori antibodies (Lower, first three panels). Also shown is staining with secondary antibody (Histofine Simple Stain MAX-PO[R] Kit) alone (Upper and Lower, panel 4). Immunocytochemistry of H. pylori (ATCC 43504) incubated in Brucella broth was supplemented with 10% inactivated horse serum at 35 °C in 15% CO₂ for 24 h with anti-CGL antibody preincubated with 0.75 μM of oxidized CGL as hapten is shown as antigen adsorption (Upper, panel 5) (Scale bar, 2 μm).