Fig. 1.

Investigating the cooperativity of sequences evolved under zero, low, medium, and high values of the tuning coefficient $\eta$ by considering (A) the fraction of the system found in the fully unfolded state during denaturation under increased temperature; (B) the fraction in the ensemble of intermediate states during the unfolding transition; (C) heat capacity curves during the unfolding transition, the area under which is the enthalpy change associated with the transition; and (D) the van’t Hoff ratio of the unfolded transition associated with each value of the cooperativity tuning coefficient $\eta$. All values are averaged over the set of 1,000 most evolved sequences for each evolutionary simulation.