TABLE 1.
List of cerebellar proteins differentially expressed according to feeding conditions and/or time-of-day.
| Spot N° | Protein name | Gene name | Subcellular location | Related functions | Effect | |
| 537 | RAC-beta serine/threonine-protein kinase | Akt2 | Plasma membrane, nucleus, endosome | Metabolism | Feeding | Increased |
| 279 | Delta-aminolevulinic acid dehydratase | Alad | Cytosol, nucleus | Miscellaneous | Feeding | Increased |
| 1,050 | Cytosolic 10-formyltetrahydrofolate dehydrogenase | Aldh1l1 | Cytoplasm | Metabolism | Feeding | Decreased |
| 1,023 | Fructose-bisphosphate aldolase C | Aldoc | Cytosol | Carbohydrate metabolism | Feeding | Increased |
| 245 | Annexin A5 | Anxa5 | Cytosol | Synapse and trafficking | Feeding | Decreased |
| 453 + 454 | ATP synthase subunit beta, mitochondrial | Atp5f1b | Mitochondrion | Mitoch. function, Energy metab. | Feeding | Decreased |
| 583 | V-type proton ATPase catalytic subunit A | Atp6v1a | Cytoplasm | Synapse and trafficking | Feeding | Decreased |
| 493 | V-type proton ATPase subunit B, brain isoform | Atp6v1b2 | Membrane | Synapse and trafficking | Feeding | Decreased |
| 1,244 | Carbonic anhydrase-related protein | Ca8 | Cytoplasm | Miscellaneous | Feeding | Decreased |
| 536 | Calreticulin | Calr | Endoplasmic reticulum, cytosol | Protein metabolism | Feeding | Decreased |
| 544 + 1,145 | T-complex protein 1 subunit zeta | Cct6a | Cytoplasm | Protein metabolism | Feeding | Decreased |
| 29 | Cytochrome c oxidase subunit 5B, mitochondrial | Cox5b | Mitochondrion | Mitoch. function, Energy metab. | Feeding | Increased |
| 544 | Dihydropyrimidinase-related protein 1 | Crmp1 | Cytoplasm, cytoskeleton | Synapse and trafficking | Feeding | Decreased |
| 21 | D-dopachrome decarboxylase | Ddt | Cytoplasm | Miscellaneous | Feeding | Increased |
| 620 | ATP-dependent RNA helicase DDX3X | Ddx3x | Nucleus, cytosol, membrane | Nucleotide metabolism | Feeding | Increased |
| 620 | ATP-dependent RNA helicase DDX3Y | Ddx3y | Nucleus, cytosol | Nucleotide metabolism | Feeding | Increased |
| 558 + 537 | Dihydropyrimidinase-related protein 2 | Dpysl2 | Cytoskeleton, cytosol | Synapse and trafficking | Feeding | Increased |
| 537 | Dihydropyrimidinase-related protein 3 | Dpysl3 | Cytoplasm | Synapse and trafficking | Feeding | Increased |
| 537 | Cytoplasmic dynein 1 light intermediate chain 1 | Dync1li1 | Cytoskeleton, cytosol | Synapse and trafficking | Feeding | Increased |
| 544 | EH domain-containing protein 1 | Ehd1 | Membrane, endosome | Synapse and trafficking | Feeding | Decreased |
| 42 | Fatty acid-binding protein, brain | Fabp7 | Cytoplasm | Lipid transport | Feeding | Decreased |
| 493 | Peptidyl-prolyl cis-trans isomerase FKBP4 | Fkbp4 | Cytoplasm, mitochondrion, nucleus | Protein metabolism | Feeding | Decreased |
| 537 | Glucose-6-phosphate 1-dehydrogenase X | G6pdx | Cytosol, nucleus | Carbohydrate metabolism | Feeding | Increased |
| 208 | Glutamate–cysteine ligase regulatory subunit | Gclm | Cytosol | Glutathione metabolism | Feeding | Decreased |
| 1,100 | Glutamine synthetase | Glul | Cytoplasm (mainly) | Amino acid metabolism | Feeding | Increased |
| 318 | Guanine nucleotide-binding protein G(o) subunit alpha | Gnao1 | Plasma membrane | Synapse and trafficking | Feeding | Decreased |
| 29 | Histone H4 | Hist1h4a | Nucleus | Chromosome stability | Feeding | Increased |
| 741 | Endoplasmin | Hsp90b1 | Endoplasmic reticulum | Protein metabolism | Feeding | Decreased |
| 583 | Heat shock-related 70 kDa protein 2 | Hspa2 | Cytoplasm, Cytoskeleton | Protein metabolism | Feeding | Decreased |
| 771 | Heat shock 70 kDa protein 4 | Hspa4 | Cytoplasm | Protein metabolism | Feeding | Decreased |
| 583 | Heat shock cognate 71 kDa protein | Hspa8 | Membrane, nucleus | Protein metabolism | Feeding | Decreased |
| 583 | Stress-70 protein, mitochondrial | Hspa9 | Mitochondrion, nucleus | Mitochondrial function | Feeding | Decreased |
| 517 | 60 kDa heat shock protein, mitochondrial | Hspd1 | Mitochondrion | Mitochondrial function | Feeding | Increased |
| 680 + 681 | MICOS complex subunit Mic60 | Immt | Mitochondrion | Mitochondrial function | Feeding | Increased |
| 517 | Alpha-internexin | Ina | Cytoskeleton | Synapse and trafficking | Feeding | Increased |
| 279 | LIM and SH3 domain protein 1 | Lasp1 | Cytoplasm, cytoskeleton | Synapse and trafficking | Feeding | Increased |
| 843 | Leucine-rich PPR motif-containing protein, mitochondrial | Lrpprc | Mitochondrion, nucleus | Protein metabolism | Feeding | Increased |
| 639 | NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial | Ndufs1 | Mitochondrion | Mitoch. function, Energy metab. | Feeding | Decreased |
| 630 + 625 + 620 | Vesicle-fusing ATPase | Nsf | Cytosol, Golgi apparatus | Synapse and trafficking | Feeding | Increased |
| 245 | Ubiquitin thioesterase OTUB1 | Otub1 | Cytoplasm | Protein metabolism | Feeding | Decreased |
| 843 | Pyruvate carboxylase, mitochondrial | Pc | Mitochondrion | Mitoch. function, carbohydrate metabolism | Feeding | Increased |
| 392 + 1,100 | Pyruvate dehyd. E1 component subunit alpha, mitochondrial | Pdha1 | Mitochondrion | Mitoch. function, Energy metab. | Feeding | Increased |
| 537 | Protein disulfide-isomerase A3 | Pdia3 | Endoplasmic reticulum | Protein metabolism | Feeding | Increased |
| 208 | Prohibitin | Phb | Mitochondrion, nucleus, membrane | Mitochondrial function | Feeding | Decreased |
| 1,244 | DNA-directed RNA polymerase II subunit RPB3 | Polr2c | Nucleus | Nucleotide metabolism | Feeding | Decreased |
| 279 | Serine/threonine-protein phosphatase PP1-beta catalytic subunit | Ppp1cb | Cytoplasm, nucleus | Miscellaneous | Feeding | Increased |
| 279 | Serine/threonine-protein phosphatase PP1-gamma catalytic subunit | Ppp1cc | Cytoplasm, mitochondrion, nucleus | Miscellaneous | Feeding | Increased |
| 202 | Proteasome subunit beta type-7 | Psmb7 | nucleus, cytosol | Protein metabolism | Feeding | Decreased |
| 537 | Serine–tRNA ligase, cytoplasmic | Sars | Cytoplasm, nucleus | Protein metabolism | Feeding | Increased |
| 339 | Neuronal-specific septin-3 | Sept3 | Cytoskeleton | Synapse and trafficking | Feeding | Increased |
| 493 | Septin-4 | Sept4 | Cytoplasm, cytoskeleton | Synapse and trafficking | Feeding | Decreased |
| 1,023 | Septin-5 | Sept5 | Cytoskeleton | Synapse and trafficking | Feeding | Increased |
| 1,091 | Leukocyte elastase inhibitor A | Serpinb1a | Cytoplasm | Protein metabolism | Feeding | Decreased |
| 318 | Endophilin-A1 | Sh3gl2 | Endosome | Synapse and trafficking | Feeding | Decreased |
| 594 + 600 | Calcium-binding mitochondrial carrier protein Aralar1 | Slc25a12 | Mitochondrion | Mitochondrial function | Feeding | Increased |
| 453 | Tubulin alpha-1B chain | Tuba1b | Cytoskeleton | Synapse and trafficking | Feeding | Decreased |
| 453 | Tubulin beta-3 chain | Tubb3 | Cytoskeleton | Synapse and trafficking | Feeding | Decreased |
| 453 | Tubulin beta-4A chain | Tubb4a | Cytoskeleton | Synapse and trafficking | Feeding | Decreased |
| 453 | Tubulin beta-4B chain | Tubb4b | Cytoskeleton | Synapse and trafficking | Feeding | Decreased |
| 453 | Tubulin beta-5 chain | Tubb5 | Cytoskeleton | Synapse and trafficking | Feeding | Decreased |
| 1,100 | Elongation factor Tu, mitochondrial | Tufm | Mitochondrion | Protein and nucleotide metab. | Feeding | Increased |
| 382 | Alpha-centractin | Actr1a | Cytoskeleton | Synapse and trafficking | Feeding + Interaction | Increased + RF > AL ZT5 |
| 382 | Beta-centractin | Actr1b | Cytoskeleton | Synapse and trafficking | Feeding + Interaction | Increased + RF > AL ZT5 |
| 382 + 392 | Glutamine synthetase | Glul | Cytoplasm (mainly) | Amino acid metabolism | Feeding + Interaction | Increased + RF > AL ZT5 |
| 382 | Phosphoglycerate kinase 1 | Pgk1 | Cytoplasm | Carbohydrate metabolism | Feeding + Interaction | Increased + RF > AL ZT5 |
| 541 | Protein disulfide-isomerase A3 | Pdia3 | Endoplasmic reticulum | Protein metabolism | Feeding + Time | Decreased + ZT5 < ZT17 |
| 610 | ATP synthase subunit beta, mitochondrial | Atp5f1b | Mitochondrion | Mitoch. function, Energy metab. | Feeding + Time | Decreased + ZT5 < ZT17 |
| 1,085 + 1,087 | Heat shock protein HSP 90-alpha | Hsp90aa1 | Membrane, nucleus | Protein metabolism | Feeding + Time | Decreased + ZT5 < ZT17 |
| 1,085 + 1,087 | Heat shock protein HSP 90-beta | Hsp90ab1 | Membrane, nucleus | Protein metabolism | Feeding + Time | Decreased + ZT5 < ZT17 |
| 610 | Endoplasmic reticulum chaperone BiP | Hspa5 | Endoplasmic reticulum | Protein metabolism | Feeding + Time | Decreased + ZT5 < ZT17 |
| 541 | T-complex protein 1 subunit epsilon | Cct5 | Cytoskeleton, cytoplasm | Protein metabolism | Feeding + Time | Decreased + ZT5 < ZT17 |
| 610 | Spectrin alpha chain, non-erythrocytic 1 | Sptan1 | Cytoplasm, Cytoskeleton | Synapse and trafficking | Feeding + Time | Decreased + ZT5 < ZT17 |
| 53 | Ubiquitin-conjugating enzyme E2 N | Ube2n | Nucleus | Protein metabolism | Interaction | RF > AL ZT5 + RF < AL ZT17 |
| 82 | DNA-directed RNA polymerases I, II, and III subunit RPABC3 | Polr2h | Nucleus | Nucleotide metabolism | Interaction | RF > AL ZT5 + RF < AL ZT17 |
| 82 | Beta-synuclein | Sncb | Cytoplasm, synapse | Synapse and trafficking | Interaction | RF > AL ZT5 + RF < AL ZT17 |
| 105 | Visinin-like protein 1 | Vsnl1 | Cytosol | Synapse and trafficking | Interaction | RF > AL ZT5 + RF < AL ZT17 |
| 139 | Proteasome subunit beta type-4 | Psmb4 | Nucleus, cytosol | Protein metabolism | Interaction | RF > AL ZT5 |
| 155 | Ras-related protein Rab-11B | Rab11b | Synapse, endosome | Synapse and trafficking | Interaction | RF < AL ZT5 |
| 155 | Ras-related protein Rab-5C | Rab5c | Plasma membrane, endosome | Synapse and trafficking | Interaction | RF < AL ZT5 |
| 216 | Eukaryotic translation initiation factor 4H | Eif4h | Cytoplasm | Protein metabolism | Interaction | RF > AL ZT5 |
| 219 | Proteasome subunit alpha type-1 | Psma1 | Nucleus, cytosol | Protein metabolism | Interaction | RF > AL ZT17 |
| 317 | Guanine nucleotide-binding protein G(o) subunit alpha | Gnao1 | Plasma membrane | Synapse and trafficking | Interaction | |
| 317 | Endophilin-A1 | Sh3gl2 | Endosome | Synapse and trafficking | Interaction | |
| 523 | Protein disulfide-isomerase A3 | Pdia3 | Endoplasmic reticulum | Protein metabolism | Interaction | RF < AL ZT5 |
| 523 | Serine/threonine-protein phosphatase 5 | Ppp5c | Plasma membrane, cytoplasm, nucleus | Miscellaneous | Interaction | RF < AL ZT5 |
| 554 | Heterogeneous nuclear ribonucleoprotein K | Hnrnpk | Cytoplasm, nucleus | Protein and nucleotide metab. | Interaction | |
| 575 | Dihydropyrimidinase-related protein 2 | Dpysl2 | Cytoskeleton, cytosol | Synapse and trafficking | Interaction | ZT5 > ZT17 RF |
| 1,019 + 1,022 | Hemoglobin subunit beta-1 | Hbb-b1 | [Red blood cells] | Miscellaneous | Interaction | ZT5 > ZT17 RF |
| 36 | Parvalbumin alpha | Pvalb | Cytosol, nucleus | Synapse and trafficking | Time + Interaction | ZT5 < ZT17 AL, RF intermediate |
| 553 | Heterogeneous nuclear ribonucleoprotein K | Hnrnpk | Cytoplasm, nucleus | Protein and nucleotide metab. | Time + Interaction | ZT5 > ZT17 RF |
| 158 | Glutamine amidotransferase-like class 1 domain-containing protein 3A, mitochondrial | Gatd3a | Mitochondrion | Mitochondrial function | Time-of-day | ZT5 < ZT17 |
| 158 | Glutathione S-transferase Mu 1 | Gstm1 | Cytoplasm | Glutathione metabolism | Time-of-day | ZT5 < ZT17 |
| 158 | Protein-L-isoaspartate(D-aspartate) O-methyltransferase | Pcmt1 | Cytoplasm | Protein metabolism | Time-of-day | ZT5 < ZT17 |
| 512 | Glucose-6-phosphate 1-dehydrogenase X | G6pdx | Cytosol, nucleus | Carbohydrate metabolism | Time-of-day | ZT5 > ZT17 |
| 512 | Synapsin-2 | Syn2 | Cytoskeleton, nucleus | Synapse and trafficking | Time-of-day | ZT5 > ZT17 |
| 550 | Heterogeneous nuclear ribonucleoprotein K | Hnrnpk | Cytoplasm, nucleus | Nucleotide and Protein metabolism | Time-of-day | ZT5 > ZT17 |
| 1,020 | Hemoglobin subunit beta-1 | Hbb-b1 | [Red blood cells] | Miscellaneous | Time-of-day | ZT5 > ZT17 |
| 1,218 | Vimentin | Vim | Cytoskeleton, nucleus, endoplasmic reticulum, mitochondria | Synapse and trafficking | Time-of-day | ZT5 > ZT17 |
| 1,331 | Glutamate dehydrogenase 1, mitochondrial | Glud1 | Mitochondrion | Mitochondrial function | Time-of-day | ZT5 < ZT17 |
| 1,330 + 1,331 | ATP synthase subunit alpha, mitochondrial | Atp5f1a | Mitochondrion | Mitoch. function, Energy metab. | Time-of-day | ZT5 < ZT17 |
Listed are the proteins identified in the proteins spots of which intensity changes between mouse groups highlighted significant effects of feeding conditions [restricted feeding (RF) vs control conditions: food ad libitum + nighttime feeding on day of sampling (AL)], time-of-day (Zeitgeber ZT5 and ZT17), and their interactions. For Feeding effects, the way protein abundance was changed in RF vs AL is given; for time-of-day and interaction effects, the groups that were different are specified in the right column. Subcellular location is based on annotations from the UnitProtKB database.