Figure 1.

(A) Experimental setup. 32 BALB/C 12-week-old mice were applied a full thickness necrosis by burning under analgesia/anesthesia and infected with pseudomonas aeruginosa embedded in alginate to mimic a biofilm. The mice were randomized into three treatment groups: 1) Ciprofloxacin and S100A8/A9 (n=14). 2) Ciprofloxacin monotherapy and placebo saline (n=12). 3) Saline controls (n=6). Half of the mice in the three groups were terminated day 10 post-burn (day 6 of infection) and the remaining on day 14 (day 10 of infection). All mice were treated until their sacrifice. (B) Wounds were photographed during and after sacrifice for wound size evaluation. Wounds were collected and homogenized for further quantitative bacteriology, host factor measurements by means of Luminex and resistance testing. Bacteria from three mice wounds developed Pseudomonas resistance by Etest. These were further processed with pulsed field electrophoresis and whole genome sequencing (Created with BioRender.com).