Skip to main content
. 2021 Mar 9;3(2):fcab024. doi: 10.1093/braincomms/fcab024

Figure 4.

Figure 4

Parietal white matter histological outcomes. Top panels: Coronal sections showing the parietal lobe stained with Iba-1 taken at ×2.5 magnification (scale bar = 1000 µm). Note the transient nature of microglial aggregates (arrows) within the sham groups (A−D) compared with the profound changes seen throughout the entire parietal lobe in the UCO group (E−H). Middle panels (I−L): graphs showing changes in myelination quantified as area fraction of MBP positive staining after sham occlusion (white columns) and UCO (grey columns). Three-way ANOVA including time and the three regions (IGWM1/2 and PVWM) as repeated measures showed a significant reduction in MBP positive myelin in the UCO groups within the parietal lobe regions (P < 0.05). Two-way ANOVA including time as a repeated measure showed a significant reduction in MBP positive myelin in the UCO groups within the peri-lesion region of the temporal lobe (P < 0.05). Statistical significance shown on the figure was determined on individual time-points by one-way ANOVA for the peri-lesion region (#P < 0.05 sham versus UCO). Data are means ± SEM. N = Sham/UCO, MBP: 3d 7/8, 7d 7/8, 14d 9/8, 21d 9/9. Bottom panels: ×2.5 magnification images (M, O) of the parietal lobe stained with MBP with representative ×20 images (N, P). Note that the MBP positive myelin appeared morphologically similar between sham and UCO groups at 21 days (Scale bars = 1000 µm for ×2.5 and 200 µm for ×20 images).