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. 2021 Mar 9;3(2):fcab024. doi: 10.1093/braincomms/fcab024

Figure 5.

Figure 5

Microglia in non-cystic regions. Left panels (A−P): representative photomicrographs of Iba-1-positive microglia in the PVWM (parietal lobe) and peri-lesion region (temporal lobe). Inserts show higher magnification of microglial morphology. Note the profound morphology and density changes between sham and UCO groups, especially in the peri-lesion region at 3 days (C versus D) and 14 days (K versus L). All images were taken at ×40 magnification (inserts are additional ×2 magnification). Scale bar = 100 µm. Right panels (Q-T): numbers of Iba-1-positive microglia after sham occlusion (white columns) and UCO (grey columns). Three-way ANOVA including time, group and the three regions (IGWM1/2 and PVWM) as repeated measures showed a significant reduction in Iba1 positive microglia in the UCO groups within the parietal lobe regions (P < 0.05). Statistical significance shown on the figure was determined on individual time-points by one-way ANOVA for the peri-lesion region (#P < 0.05 sham versus UCO) and two-way ANOVA for the parietal lobe with area (IGWM1/2 and PVWM) as a repeated measure (*P < 0.05 sham versus UCO). Data are means ± SEM. N = Sham/UCO, Iba-1: 3d 9/8, 7d 8/8, 14d 9/8, 21d 9/8.