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. 2021 Apr 18;13(4):1349. doi: 10.3390/nu13041349

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Effect of GJ on pathological changes to the intestine in ABX-induced AD mice. (A) Rectal bleeding was measured with a Hemoccult Sensa^® kit. Blue coloration indicates rectal hemorrhage. (B) Score table proposed based on manufacturer’s guidelines. (C) Occult blood DAI and (D) stool consistency DAI were evaluated. (E,F) The length of the intestine of each group was measured. (G) H&E staining (magnification ×100) was performed to evaluate intestinal villi length and (H) histological scoring of intestinal injury was evaluated. Vehicle: normal control group; ABX: antibiotic cocktail-administered group; DNCB: DNCB-applied group; ABX+DNCB: antibiotic cocktail-administered and DNCB-applied group; A+D+GJ, antibiotic cocktail-administered and DNCB-applied group fed with GJ. ** p < 0.01, *** p < 0.005 and **** p < 0.001.