Figure 5.

Further validation with MDN. (a) Linear regression performed on results of summed neurite length per image quantified with the presented approach and manual analysis using NeuronJ. ($y=0.3403·x+2109$; $R^2=0.9297$) (b) Representative micrographs showing a selection of experimental conditions (positive control (CTRL) and 10 nM and 40 nM Rotenone). Stained against TH (green). Cell nuclei were stained with DAPI (blue). Scale bar = 100 µm. (c) Linear regression performed on results of neurite length normalized to the number of TH${}^{+}$-cells and afterwards to positive control. ($y=0.9605·x+0.1517$; $R^2=0.8296$) (d) Neurotoxic effects of Rotenone on the neurite network of MDN quantified with manual analysis. Mean neurite length per cell normalized to TH${}^{+}$-cells. (e) Linear regression performed on results of neurite length normalized to cell count and afterwards to positive control. ($y=0.8460·x+0.2482$; $R^2=0.7959$) (f) showing the neurotoxic effects of Rotenone quantified using the presented neurite outgrowth quantification assay. Mean neurite length per cell normalized to TH${}^{+}$-cells. Data are shown as mean ± SM. * Significantly different ($p<0.05$) between mean of solvent control (DMSO) and mean of according Rotenone treatment condition. Respectively ** with $p<0.01$, *** with $p<0.001$ and **** with $p<0.0001$.