Table 1.
Antibodies for flow cytometry.
| Antigen | Conjugate | Clone | Source | Staining | Panels | |||
|---|---|---|---|---|---|---|---|---|
| CD11b | BV421 | M1/70 | BioLegend | Surface | 1 | |||
| CD11c | APC-Cy7 | N418 | BioLegend | Surface | 1 | |||
| CD25 | APC | PC61.5 | eBiosciences | Surface | 1 | 1 | 1 | |
| CD3 | APC-Cy7 | 145-2C11 | BioLegend | Surface | 1 | 1 | 1 | |
| CD317 | APC | 927 | BioLegend | Surface | 1 | |||
| CD4 | BV5101/PerCP-Cy5.52 | RM4-5 | BioLegend | Surface | 1 | 2 | 2 | |
| CD45 | Alexa Fluor 700 | 30-F11 | BioLegend | Surface | 1 | 1 | 1 | 1 |
| CD73 | BV605 | TY/11.8 | BioLegend | Surface | 1 | |||
| CD8 | FITC | 53-6.7 | BD Biosciences | Surface | 1 | 1 | ||
| CTLA-4 | PE/Dazzle594 | UC10-4B9 | BioLegend | Surface | 1 | 1 | ||
| F4/80 | BV510 | BM8 | BioLegend | Surface | 1 | |||
| Foxp3 | PE | FJK-16s | eBiosciences | Nuclear | 1 | 1 | 1 | |
| GITR | FITC | DTA-1 | BioLegend | Surface | 1 | |||
| Granzyme B | PE/Cy7 | NGZB | BioLegend | Intracellular | 1 | 1 | ||
| ICOS | BV650 | 7E.17G9 | BioLegend | Surface | 1 | |||
| IFN-γ | BV421 | XMG1.2 | BioLegend | Intracellular | 1 | |||
| IL-17A | BV650 | TC11-18H10.1 | BioLegend | Intracellular | 1 | |||
| IL-4 | BV786 | 11B11 | BD Biosciences | Intracellular | 1 | |||
| Lag3 | BV785 | C9B7W | BioLegend | Surface | 1 | |||
| Ly6C | BV786 | HK1.4 | BioLegend | Surface | 1 | |||
| Ly6G | FITC | 1A8 | BioLegend | Surface | 1 | |||
| MHC-II | PE-Cy7 | 114.15.2 | BioLegend | Surface | 1 | |||
| NK1.1 | BV650 | PK136 | BioLegend | Surface | 1 | |||
| Nrp-1 | BV 421 | 3E12 | BioLegend | Surface | 1 | |||
| PD-1 | PE-Cy71/PE2 | 29F.1A12 | BioLegend | Surface | 1 | 2 | ||
| PD-L1 | PE-DAZZLE 594 1/BV650 2 |
10F.9G2 | BioLegend | Surface | 1 | 2 | ||
| TCR γ/δ | BV605 | GL3 | BioLegend | Surface | 1 | 1 | ||
1,2—antibody present in a panel.