Figure 2.

The effects of AdPLA siRNA transfection on the expression of AdPLA in the HPA-s and 3T3-L1 cells. HPA-s cells and 3T3-L1 cells were transfected with human and mouse AdPLA siRNAs, respectively, or the corresponding scrambled siRNAs, at 24 h after transfection, (a and b) the mRNA expression levels of AdPLA in HPA-s and 3T3-L1 cells were determined by qRT-PCR, and (c, d) the protein expression levels of AdPLA in HPA-s and 3T3-L1 cells were determined by western blot. Data were presented as mean ± standard deviation; n = 3. *P < .05 (unpaired Student’s t-test).