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. 2021 Apr 19;10(4):621. doi: 10.3390/antiox10040621

Figure 3.

Figure 3

Effect of exercise and the combined action of exercise and catechin treatment on TPH-2 enzyme activity in the hippocampus (A) and striatum (B), and the effect on TPH-1 enzyme activity in the pineal gland (C) of aged rats. Bars represent the mean ± SEM of 5-HTP accumulation (after inhibition of decarboxylation with NSD-1015 for 30 min) (A1,B1,C1), 5-HT levels (A2,B2,C2) and 5-HIAA metabolite (A3,B3,C3) in extracts from the hippocampus and striatum (ng/g) and ng in the entire pineal gland (ng/pineal) of animals subjected to exercise (Exerc, n = 7) and the exercise plus catechin treatment group (Exerc + Cat, n = 7) versus control group animals (n = 7). (A) One-way ANOVA detected a significant effect on 5-HTP (F(2,18) = 100.2, p < 0.0001), 5-HT (F(2,18) = 61.74, p < 0.0001) and 5-HIAA (F(2,18) = 3.425, p = 0.05) in the hippocampus. (B) One-way ANOVA detected a significant effect on 5-HTP (F(2,18) = 141.2, p < 0.0001) and 5-HT (F(2,18) = 48.73, p < 0.0001), but not 5-HIAA (F(2,18) = 1.232, p = 0.314) in the striatum. (C) One-way ANOVA detected a significant effect on 5-HTP (F(2,16) = 23.6, p < 0.0001) and 5-HT (F(2,16) = 14.9, p = 0.0003), but not 5-HIAA (F(2,16) = 0.153, p = 0.859) in the pineal gland. ** p < 0.01, *** p < 0.001 comparing with the control group; # p < 0.05, ### p < 0.001 comparing both exercise groups with each other (one-way ANOVA, followed by Bonferroni test).