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BETi treatment downregulated ACE2 transcript levels in various cell types. Gene expression of hACE2 (A,C–E) or RhACE2 (B) was measured by TaqMan real-time PCR. Calu-3 (A) were treated for 48 h, Vero E6 (B) were treated for 24 h, HepG2 (C), and Huh-7 (D) cells were treated for 96 h. Primary human hepatocytes (n = 3 donors) (E) were treated for 48 h. Error bars represent SD. * p < 0.05, ** p < 0.01, *** p < 0.001, one-way ANOVA followed by Dunnett’s multiple comparison test.
