Figure 6.

Challenge of vaccinated mice with a highly lethal dose of H1N1 virus. Mice were vaccinated with each of the indicated NA constructs (n = 5 per group) in a prime boost regimen. Here, 3 ug of the respective recombinant protein adjuvanted with AddaVax was administered. As negative and positive controls, the Lassa virus glycoprotein and the tetrameric Mich15-VASP protein were administered. Mice were then challenged with 100× LD₅₀ of A/Singapore/GP1908/2015. Weight loss was monitored over 14 days post-challenge (A). Mice were euthanized when they lost 25% of their initial weight. Survival curves are shown (B).