Figure A5.

Effects of SPION^HAp on the viability of Jurkat cells (ACC 282). Cells were incubated with SPION^HAp particle concentrations of 25 to 400 μg/mL. After 48 h, cells were stained with Annexin A5 FITC conjugate (AxV) and propidium iodide (PI) to detect apoptotic and necrotic cells by flow cytometry. Viable cells (AxV- PI-) are visualized in green, apoptotic cells (AxV+ PI-) in blue, and necrotic cells (PI+) in red. The experiments were performed in triplicate. The mean values are shown with SD. Significance between treatment groups and control (viable cells) at the respective time is indicated by asterisks: * p < 0.05. p values higher than 0.05 were assumed to be not significant (n.s.).