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. 2021 Feb 18;7(3):271–286. doi: 10.1002/cjp2.205

Figure 4.

Figure 4

ASPN and NT5E expression in cribriform prostate cancer. (A) Representative images of dual ASPN (blue) and NT5E (red) mRNA expression in stroma adjacent to benign prostate (n = 9), Gleason grade 3 prostate cancer (n = 6), Gleason grade 4 prostate cancer without cribriform morphology (n = 6), and Gleason grade 4 prostate cancer with cribriform morphology (n = 6) (×400 magnification). (B) The percentage of NT5E + stromal cells adjacent to benign prostate, Gleason grade 3 prostate cancer, Gleason grade 4 prostate cancer without cribriform morphology, and Gleason grade 4 prostate cancer with cribriform morphology. (C) The relative expression of NT5E in NT5E + stromal cells. (D) The mean NT5E H‐score. (E) The percent ASPN + NT5E −, ASPN − NT5E +, and ASPN + NT5E + stromal cells adjacent to benign prostate, Gleason grade 3 prostate cancer, Gleason grade 4 prostate cancer without cribriform morphology, and Gleason grade 4 prostate cancer with cribriform morphology. Gene expression detected by RNAScope® and quantified with Halo® Software. Statistical analyses performed using one‐way analysis of variance with Tukey multiple comparisons. Graphs shown as mean ± SEM, *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001.